Biomass production under well-watered growth regimes or drought. (A) RCAR6-3 (R6) and RCAR10-4 (R10) consistently had a higher biomass yield than Col-0 under progressively drying soil (see Fig. 3 for drought condition). The mean above-ground biomass of Col-0 was 0.31 g, 0.31 g, and 0.27 g in three independent experiments (numbered 1–3) and the yield of RCAR lines is expressed relative to Col-0 (n = 4 per data point, mean ± SEM, **P < 0.001 compared with wild type). (B) The dry weight of above-ground biomass of Arabidopsis wild type Col-0 (n = 28), RCAR6-3 (R6, n = 28), and RCAR10-4 (R10, n = 4) was determined after 4 mo of growth under well-watered and long-day regimes (16 h of light per day). Above-ground biomass of Col-0 was 1.41 ± 0.11 g and set to 100%. (C) Representative pictures of the three lines at the age of 8 wk (Upper row) and of rosettes 7 wk (Lower) grown under long-day and short-day, respectively, and under well-watered conditions.