Fig. S4.

Gas exchange analysis of wild-type Col-0 and RCAR6 overexpressing plant. (A and B) Analysis of the leaf rosette of Arabidopsis plants for the relation of the soil water content (vol/vol) and (A) the ratio of net CO₂ assimilation (A_n) and stomatal conductance (g_s), which reflects the partial CO₂ pressure gradient at the stomatal pore, and (B) g_s in Col-0 (filled circle) and the RCAR6-3 line (open square). (C and D) Single leaves of Arabidopsis were analyzed for net CO₂ assimilation rates at (C) different light intensities and ambient CO₂ (C_a = 400 μmol⋅mol⁻¹) as well as for (D) different atmospheric CO₂ levels (C_a) and a constant photon flux of 150 μmol⋅m⁻²⋅s⁻¹, both at well-watered conditions (Ψ > −0.03 bar). In C, leaves were preadjusted to saturating light intensities (1,500 μmol⋅m⁻²⋅s⁻¹) for 2 h before the analysis. (A and B) Single plant measurements with 10 technical replicates per data point. (C and D) n = 3 biological replicates per line, four technical replicates per data point, mean ± SEM.