Table S1.

Strains used in this study

No.	Strain	Characteristic	Source
E. coli
3044	DH5αλpir	sup E44, ΔlacU169 (ΦlacZΔM15), recA1, endA1, hsdR17, thi-1, gyrA96, relA1, λpir phage lysogen	V. de Lorenzo
P. putida
1291	UWC1	Plasmid-free derivative of P. putida KT2440, RifR	Ref. 49
2737	UWC1-clc5	Derivative of strain 1291 with one ICEclc copy integrated into tRNAgly-5	Ref. 40
4322	UWC1-clc5 ΔmfsR	Derivative of strain 2737 with an internal deletion in mfsR	Ref. 37
4611	UWC1-clc5 ICEclc-intB13-gfp	Derivative of strain 2737 with a promoterless KmR gene inserted 27 bp downstream of intB13	This study
4612	UWC1-clc5 ICEclc-intB13-gfp, ∆mfsR	Derivative of strain 4322 with a promoterless egfp gene inserted 27 bp downstream of intB13	This study
4669–4672	UWC1-clc5 ICEclc-intB13-gfp, Tn5 PinR-echerry	Derivatives of strain 4611 with random chromosomal insertion of miniTn5 PinR-echerry. Four independent clones, KmR	This study
4673–4676	UWC1-clc5 ICEclc-intB13-gfp, ∆mfsR, Tn5 PinR-echerry	Derivatives of strain 4612 with random chromosomal insertion of miniTn5 PinR-echerry. Four independent clones, KmR	This study
4754	UWC1-clc5 ICEclc-intB13-gfp, ∆attL	Derivative of 4611 with 18 bp attL deletion	This study
4755	UWC1-clc5 ICEclc-intB13-gfp, ∆mfsR, ∆attL	Derivative of 4612 with 18 bp attL deletion	This study
2744	UWC1GC	Derivative of strain 1291 with single copy miniTn7 Ptac-echerry, GmR	Ref. 29
2756	UWC1 miniTn5 JimX-gfp	Derivative of UWC1 with miniTn5 insertion of the jimX-gfp fragment, KmR	Ref. 40
5001–5003	UWC1 miniTn5 JimX-echerry	Derivatives of UWC1 with miniTn5 insertion of the jimX-echerry fragment, KmR	This study