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. 2016 Feb 22;7(11):11756–11769. doi: 10.18632/oncotarget.7598

Figure 3. MUC1 increases xCT stability.

Figure 3

A. 293T cells were transfected to stably express MUC1-C or a control vector. Lysates from the transduced cells were immunoblotted with the indicated antibodies (left). Lysates from 293T/MUC1-C cells were precipitated with anti-MUC1-C or a control IgG. The precipitates were immunoblotted with the indicated antibodies (right). B. Membrane fractions (left) and total cell lysates (right) from 293T/vector or 293T/MUC1-C cells were immunoblotted with the indicated antibodies. C. 293T/vector and 293T/MUC1-C cells were exposed to 50 μg/ml CHX for the indicated times. Total cell lysates were immunoblotted with the indicated antibodies. D. Intensities of the xCT signals as compared to those obtained for β-actin (xCT/β-actin ratio) for the CHX-treated 293T/vector and 293T/MUC1-C cells are plotted relative to the control (time 0; assigned a value of 1). E. MDA-MB-468/CshRNA and MDA-MB-468/MUC1shRNA cells were exposed to CHX (50 μg/ml) for the indicated times. Total cell lysates were immunoblotted with the indicated antibodies. F. Intensities of the xCT signals as compared to those obtained for β-actin (xCT/β-actin ratio) for the CHX-treated MDA-MB-468/CshRNA and MDA-MB-468/MUC1shRNA cells are plotted relative to the control (time 0; assigned a value of 1).