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. 2016 Mar 2;7(11):11945–11958. doi: 10.18632/oncotarget.7858

Figure 2. Effect of TNF-α blockade on the expression of miRs and their target proteins in senescent (SA-β-Gal > 50 %) and young (SA-β-Gal < 5 %) HUVECs with and without LPS-stimulation.

Figure 2

A. TNF-α release into the culture medium by LPS-exposed (1 μg/ml) young and senescent HUVECs, expressed as pg/ml per 100,000 cells. B. MiR-146a-5p expression in young and senescent HUVECs with/without 24 h anti-TNF-α pretreatment, measured as relative expression (a.u). C. MiR-146a-5p expression in young and senescent HUVECs after 30 min or 5 h LPS stimulation, with/without 24 h anti-TNF-α pretreatment, measured as fold change vs ctrl. D. MiR-126-3p expression in young and senescent HUVECs with/without 24 h anti-TNF-α pretreatment, measured as relative expression (a.u). E. MiR-126-3p expression in young and senescent HUVECs after 30 min or 5 h LPS stimulation, with/without 24 h anti-TNF-α pretreatment, measured as fold change vs ctrl. F. Irak1 and Spred1 expression and densitometry data in young and senescent HUVECs after 30 min or 5 h LPS stimulation, with/without 24 h anti-TNF-α pretreatment. * Student's t test, p < 0.05. Data are mean ± S.D. of 3 independent experiments.

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