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. 2016 Feb 23;7(11):12063–12074. doi: 10.18632/oncotarget.7642

Figure 6. Effects of GDF11 on cell viability in HUVECs and RAECs.

Figure 6

A. Tert-Butyl hydroperoxide increased cell viability at 200μM and 300μM doses and decreased cell viability in the doses from 500 to 700μM after 6h treatment. *P < 0.05 vs. control. **P < 0.01 vs. control. B. GDF11 (50ng/ml) purchased from PeproTech slightly increased cell viability after 24h treatment and slightly decreased cell viability after 72h and 96h treatment in HUVECs. GDF11(100ng/ml) purchased from PeproTech slightly increased the cell viability in HUVEC cells at 6h and 24h and decreased the cell viability at 48h, 72h and 96h. *P < 0.05 vs. control. **P < 0.01 vs. control. C. The representative photos of live and dead cell staining. The live cells were stained with calcein AM in green, and the dead cells were stained with ethidium homodimer-1 in red. D. GDF11 purchased from R&D Systems showed no significant effect on cell viability of HUVECs. E. GDF11 purchased from PeproTech slightly decreased cell viability after 48h and 96h treatment in aortic endothelial cells (RAECs). *P < 0.05 vs. control. **P < 0.01 vs. control. F. GDF11 purchased from R&D Systems slightly decreased cell viability after 24h and 48h treatment in aortic endothelial cells (RAECs). **P < 0.01 vs. control.