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. 2016 Feb 22;7(11):12254–12266. doi: 10.18632/oncotarget.7601

Figure 1. Induction of ER stress leads to upregulation of SESTRIN 2 expression independent of P53.

Figure 1

MCF7 cells were treated with A. 0.5 μg/ml of BFA or 1 μM of Tg for the indicated time and viability assessed by propidium iodide (PI) uptake. Error bars represent the mean ± SD. MCF7 cells were treated with 0.5 μg/ml of Tg B. or 1 μM of BFA C. for the indicated time and lysates imunoblotted for SESTRIN 2, PERK, XBP1s. ACTIN was used as a loading control. D. MCF7 cells were treated with 1 μM of Tg alone or in combination with Act D (1 μg/ml) for the indicated time and lysates immunoblotted for SESTRIN 2 and ACTIN. E. MCF7 cells were treated with 0.5 μg/ml BFA, 1 μM Tg or 50 μM Etoposide (Etop) for 24 h after which lysates were immunoblotted for SESTRIN 2, Ser15 p-P53 and total P53. F. HCC1806 cells were treated with 1 μM Tg for the indicated time and cell lysates were then immunoblotted for SESTRIN 2 and ACTIN. Results are representative of at least 3 independent experiments.