Skip to main content
. 2016 Feb 22;7(11):12254–12266. doi: 10.18632/oncotarget.7601

Figure 4. Chemotherapeutics, Methotrexate and Bortezomib, induce ER stress and SESTRIN 2 expression via UPR-mediated signals.

Figure 4

A.-B. HCC1806 (A) and MCF7 (B) cells were treated with 5 mM (A) 0.5 mM (B) 5-fluorouracil (5FU), 200 μM melphalan (Mel), 200 nM (A) 100 nM (B) Taxol, 20 μM Methotrexate (Mtx), 200 nM Doxorubicin, 0.5 μM Bortezomib (Btz) for 24 h and lysates immunoblotted for SESTRIN 2 and ACTIN. C. HCC1806 cells were treated with the indicated concentrations of Mtx for 48 h and lysates immunoblotted for SESTRIN 2, PERK, XBP1s, ATF4, CHOP and ACTIN. D. HCC1806 cells were treated with 20 μM Mtx for the indicated time and lysates immunoblotted for SESTRIN 2, LC3-I/II, XBP1s, ATF4, CHOP and ACTIN. E. HCC1806 cells were treated with 20 μM Mtx for the indicated time alone or in combination with 300 nM PERK or 10 μM IRE1 inhibitor and lysates were immunoblotted for SESTRIN 2, XBP1s and ACTIN. A representative image of 3 independent experiments is shown. F. Ctrl and XBP1 siRNA-transfected HCC1806 cells were treated with 20 μM Mtx for the indicated time and lysates immunoblotted for SESTRIN 2, XBP1s and ACTIN.