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. 2016 Feb 22;7(11):12254–12266. doi: 10.18632/oncotarget.7601

Figure 7. Knockdown of SESTRIN 2 delays Mtx-induced dephosphorylation of MTOR and potentiates ER stress.

Figure 7

Ctrl and SESTRIN 2 siRNAs transfected HCC1806 cells were treated with 20 μM Mtx for the indicated time. A. Lysates were immunoblotted with SESTRIN 2, phospho-MTOR, total MTOR, phospho-P70 S6K, phospho-EIF2α, total EIF2α, ATF4, CHOP and ACTIN. B. Ctrl and SESTRIN 2 siRNAs transfected HCC1806 cells were treated 20 μM Mtx ± 100 nM Rapamycin for the indicated time. Lysates were immunoblotted for SESTRIN 2, phospho-P70 S6K, phospho-EIF2α, total EIF2α, CHOP and ACTIN. A representative image of 3 independent experiments is shown.* Denotes non-specific band.