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. 2016 Feb 3;7(11):12393–12403. doi: 10.18632/oncotarget.7161

Figure 1. The distribution of Amot in renal epithelial cells and RCC cells.

Figure 1

A. Western blot analysis of the relative levels of Amot expression in different epithelial and RCC cell lines (left panel). The specificity of anti-Amot in 786-O cells was analyzed by Western blot (right panel). B. Quantitative RT-PCR analysis of the relative levels of Amot mRNA transcripts in the different epithelial and RCC cell lines. C. Immunofluorescent analysis of Amot expression in HK-2 and 786-O cells. Scale bar = 25 μm. D. Western blot analysis of the distribution of cytoplasmic and nuclear Amot in HK-2 and 786-O cells (Nuc: nucleus; Cyto: cytoplasm). Lamin A was used as a nuclear marker. E. Immunohistochemsitry of Amot expression in human RCC and paracancerous tissues (Para: paracancerous tissues). E1, E4: anti-Amot; E2, E5: anti-Amot and antigen peptides; E3, E6: PBS control; Scale bar = 146 μm. F. Immunohistochemistry of Amot expression in human RCC and paracancerous tissues. Scale = 33μm.