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. 2016 Feb 9;7(11):12568–12581. doi: 10.18632/oncotarget.7276

Figure 2. The inhibition of ERRα induces mitochondrial-related apoptosis in TNBC cells.

Figure 2

(A) MDA-MB-231 cells were treated with 10 μM XCT-790 for increased time periods, stained with annexin V-FITC and PI, and then analyzed by FCM for cell apoptosis. (B) MDA-MB-231 cells were treated with 10 μM XCT-790 as the indicated concentrations for 24 h, and then JC-1, the mitochondria-specific dye, was added to measure the membrane polarity (ΔΨm). Apoptotic cells mainly show green fluorescence (FITC), while healthy cells show red fluorescence (PE). (C) MDA-MB-231 and BT-549 cells were treated with XCT-790 as the indicated concentrations for 48 h, and then Bcl-2, Bax, and Bim protein expression levels were analyzed by Western blot analysis. Data were presented as means ± SD of three independent experiments.