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. 2016 Feb 9;7(11):12568–12581. doi: 10.18632/oncotarget.7276

Figure 3. XCT-790 increases ROS generation by suppressing SOD1/2.

Figure 3

(A) MDA-MB-231 cells were treated with increasing concentrations of XCT-790 for 12 h, and then loaded with CM-H2DCFDA. The fluorescence intensity was measured by FCM. (B) MDA-MB-231 cells were treated with 5 μM XCT-790 for 12 h, and then the mRNA levels of SOD1, SOD2, GXP1, Catalase, NOX1, and NOX4 were measured by qRT-PCR; MDA-MB-231 cells were pretreated with or without 2000 U Catalase (C) or 10 μM DPI (D) for 1 h, and then exposed to XCT-790 for 12 h. Data were presented as means ± SD of three independent experiments.