Figure 4. Expression profiling of ELOVLs in human and mouse lung SCC.
A. mRNA levels of ELOVL1-7 were analyzed by RT-qPCR in 30 human SCC tissues and 9 pools (14-15 different samples) of matched normal tissues. A normalization factor corresponding to the geometric mean of Ct values of ESD, HMBS, POLR2A, PSMB4, TBP and UBC was used for subsequent ΔCt calculation. B. Tumor versus normal lung tissue (n=3) from the L-IKKαKA/KA SCC mouse model were collected and mRNA levels of ELOVL1-7 were measured using RT-qPCR. 18S was used for normalization. ELOVL2 and 3 were not detected. Data represent mean (log2) ± standard error. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 (Student's test).