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. 2016 Feb 3;7(11):12582–12597. doi: 10.18632/oncotarget.7179

Figure 5. Effect of knockdown of ELOVL2, 4 and 6 on acyl chain elongation in 2427PT lung SCC cells.

Figure 5

2427PT cells were transfected with siRNA specific for A. ELOVL2, B. ELOVL4 and C. ELOVL6 for 72h. Control cells were transfected with scrambled siRNA. The relative abundance and changes in relative abundance (log2) of PC after modulation of the specific ELOVL are shown. Lipid extracts were made and were subjected to MRM-based ESI-MS/MS analysis of phospholipids. Lipid species are grouped based on degree of fatty acyl chain unsaturation (groups separated by dotted lines). In each group with equal (un)saturations, the species are ordered from species with shorter fatty acyl chains to longer chains. Data represent mean (n=3) ± standard deviation. * Indicates statistical significance after multiple testing using the Holm-Sidak method with alpha=0.05.