Figure 10. Downregulation of Sox2 genes with siRNA results in increased cell proliferation.
The mRNA expression of β-catenin was increased following knockdown of the Sox2 and Sox5 genes with siRNA in a mouse Men1 WT cell line, using a real-time RT-PCR assay (A). Cell proliferation was increased following knockdown of Sox2 and Sox5 genes, with Sox2 siRNA and Sox5 siRNA in a human pancreatic tumor cell line (BON-1), using IF staining for the Ki-67 marker (B). (*P < 0.05, **P < 0.01, ***P < 0.001).