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. 2016 Feb 9;7(11):12633–12650. doi: 10.18632/oncotarget.7279

Figure 11. Increased mRNA expression of Sox genes, subsequent decreased mRNA expression of β-catenin, and decreased global DNA methylation after treatment with the demethylating agent 5-aza-2′-deoxycytidine in Men1 null MEF cells.

Figure 11

In Men1 null MEF cells, relative mRNA of Sox2, Sox3, Sox5, Sox9, Sox11, and β-catenin was measured after treatment with 1 μM 5-aza-2′-deoxycytidine for 24 h and 48 h, the asterisks indicate that the mRNA expression of Sox genes are significantly increased and the mRNA expression of β-catenin is significantly decreased as compared to controls. However, mRNA expression of Sox and β-catenin were not significantly changed in Men1 WT MEF cells after treatment (A). The global DNA methylation level is significantly decreased in Men1 null MEF cells by selective degradation DNMT1 with the demethylating agent treatment (0, fully methylated; 1, fully unmethylated) (B). (*P < 0.05, **P < 0.01, ***P < 0.001).