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. 2016 Feb 9;7(11):12633–12650. doi: 10.18632/oncotarget.7279

Figure 3. Increased expression of DNMT1 in endocrine tumor tissues from human MEN1 parathyroids and Men1 KO mice.

Figure 3

DNMT1 expression was significantly increased in the parathyroid tumors from MEN1 patients (A), mouse pancreatic endocrine tumor tissues (B), and mouse parathyroid tumor tissues (C) from Men1 KO mice compared to normal endocrine tissues from human and control mice by IF staining. DMNT1 was stained green by anti-DNMT1 antibody (Alexa Fluor 488); PTH and insulin were stained red by anti-PTH antibody or anti-insulin antibody, respectively (Alexa Fluor 647); and nuclei were stained blue with DAPI. Images were taken using a 20X objective. DNMT1 expression was determined in endocrine tumor tissues from the parathyroid in MEN1 patients (D) and pancreatic islets in Men1 KO mice (E), compared to normal endocrine tissues from humans and control mice by western blot assay (***P < 0.001). (Normal/N: human normal parathyroids; MEN1/M: human MEN1 parathyroids; KO: Men1 KO mice; WT: Men1 WT mice).