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. 2016 Feb 5;7(11):12651–12661. doi: 10.18632/oncotarget.7207

Figure 4. AR regulates miR-21 expression in breast cancer cells.

Figure 4

MDA-MB-231 cells were transfected with Vector (pcDNA3) and AR expression plasmid (pcDNA3-AR) (AR). After 24 h, cells were treated with Mib (10nM) A. for 24 h. ZR-75-1 cells were transfected with psiCon a scrambled shRNA (Vector) or psiAR (shAR) and treated as described before B.. At the end of the treatment, total RNA was extracted and analyzed by qRT-PCR to evaluate miR-21 content. All the qRT-PCR results were normalized to RNU6B. Data represent fold enrichment with respect to the correspondent untreated Vector sample and are reported as Mean ± s.d. derived from three independent measurements. Statistical analysis was performed applying Student's t test: *P < 0.05 is referred to the correspondent untreated sample; □ to the correspondent Vector samples (p < 0.05).