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. 2016 Feb 8;7(11):12682–12692. doi: 10.18632/oncotarget.7259

Figure 2. As2O3 inhibits telomerase translocation, phosphorylation and activity.

Figure 2

A. Immunofluorescent detection of the telomerase catalytic subunit (hTERT) in control U87 cells (green) and cells exposed to 4 μM As2O3 (green) or 4 μM As2O3 plus NAC (red). The translocation of hTERT from the nucleus to the cytoplasm was evidenced from the difference in hTERT accumulation. Cells were pretreated with NAC (1 mM) before As2O3 treatment. Scale bar = 25 μm. B. ROS generation induced by 0-16 μM As2O3 with and without NAC. *P < 0.05, **P < 0.01. C. Immunoblots showing the presence of hTERT in both nuclear and cytosolic extracts from cells exposed to 0-8 μM As2O3. The level of hTERT protein in the nucleus decreased as the cytoplasmic level increased. HSP70 was used as an internal standard for the cytoplasm, and topoisomerase 1 was used as an internal standard for the nucleus. The expression of hTERTTyr707 indicated the phosphorylation of telomerase. D. As2O3-induced inhibition of telomerase activity in U87 cells. TRAP assays performed after 48 h of As2O3 treatment show dose-dependent inhibition of telomerase activity. The position of the internal standard was indicated as IS. E. Dose-dependent inhibition of telomerase activity in U87, U251, SHG44 and C6 cells. *P < 0.05, **P < 0.01 as compared with controls.