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. 2016 Feb 12;7(11):12893–12903. doi: 10.18632/oncotarget.7335

Figure 3. Differential inhibition of MLN-8237 in cell lines other than sarcoma.

Figure 3

(A) Uveal melanoma cells; OCM1, OCM1A, OCM3, Mel270 and Mel290 were exposed to 0.1 and 1 μM of MLN-8237 for 48 hours and harvested for (i) western blot analysis probing for cleaved PARP, cleaved caspase 3, phospho histone H3 (Ser10), Aurora A, p21, Bim and tubulin as loading control. (ii) Fluorescent microscopic images of DAPI stained OCM3, OCM1A and OCM1 treated with 0.1 and 1 μM of MLN-8237 for 48 hours. Normal untreated (ND) (first panel), apoptotic cells (red circles, middle panel) and polyploid cells (white circles, last panel) are marked. (iii) Mel270 and Mel290 were treated with 0, 0.1 or 1 μM MLN-8237 for 48 hours were harvested for FACScan analysis after staining with propidium iodide showing cell cycle distribution. (B) HCT116 cells treated with 0, 0.1 or 1 μM MLN-8237 for 48 hours were harvested for (i) FACScan analysis after staining with propidium iodide showing cell cycle distribution showing < 2N for 0.1 and 8N for 1 μM exposure. (ii) Western blot analysis of the same treatment probed for cleaved PARP, Aurora A, pH3 (S10), Aurora B, p53, p21 and tubulin as loading control.