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. 2016 Feb 12;7(11):12975–12996. doi: 10.18632/oncotarget.7349

Figure 5. BAG2 is an AR-12 interacting protein.

Figure 5

(A) GBM5 and GBM12 cells were treated with vehicle, OSU-03012 (2.0 μM)/sorafenib (2.0 μM) and/or sildenafil (2 μM) for 6 h after which cells were fixed in place and permeabilized using 0.5% Triton X100. Immuno-fluorescence was performed to detect the expression level of BAG2 presented at 10X and 60X magnification. (B and C) GBM5 and GBM12 cells were treated with vehicle, OSU-03012 (2.0 μM)/sorafenib (2.0 μM) and/or sildenafil (2 μM) for 6 h after which cells were fixed in place and permeabilized using 0.5% Triton X100. Immuno-fluorescence was performed to detect the expression level of BAG1 and BAG3 presented at 10X magnification. (D) GBM5 and GBM12 cells were treated with vehicle, OSU-03012 (2.0 μM)/sorafenib (2.0 μM) and/or sildenafil (2 μM) for 6 h after which cells were fixed in place and permeabilized using 0.5% Triton X100. Immuno-fluorescence was performed to detect the expression level of HIP presented at 10X magnification.