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. 2016 Jan 25;7(11):12997–13012. doi: 10.18632/oncotarget.7016

Figure 3. OPN increased the stability of vimentin.

Figure 3

(A) Overexpression (left two panels) or knockdown (right two panels) of vimentin in HCC cells showed little influence on OPN expression. (B) MHCC-97L cells with stable overexpression of OPN were treated CHX (20 μg/mL) for indicated times. Endogenous vimentin protein levels were determined. MHCC-97L cells were stably transfected with empty vector used as control. (C) MHCC-97H cells transfected with shOPN or scrambled shRNA were treated MG-132 (40 μM) for indicated times. Endogenous vimentin protein levels were determined. (D) Overexpression of OPN attenuates vimentin ubiquitin. Vector control and OPN stably overexpressing MHCC-97H cells were transfected with FLAG-tagged vimentin plasmids for 24 h and treated with 40 μM MG132 for 36 hours. Cells were then harvested and lysed immunoprecipitation using anti-Flag beads.