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. 2016 Feb 21;7(11):13153–13166. doi: 10.18632/oncotarget.7549

Figure 4. Effects of BKM120 and Olaparib as single-agents and in combination on DNA damage response in ovarian cancer cells.

Figure 4

(A) DNA damage in ovarian cancer cells was determined by comet assay. The ovarian cancer cells as indicated were treated with BKM120 and Olaparib as single-agents or in combination for 48 hours. Comet images × 200 taken by fluorescent microscope were shown. Tail moment was used to quantify DNA damage and evaluated by CASP software (CaspLab). Means ± S.D. of three independent experiments are shown. (B) Representative images of immunofluorescence staining of γH2AX and RAD51 in ovarian cancer cells treated as indicated for 48 hours (left panel). Cell nuclei were stained with DAPI. The percentage of cells with γH2AX and RAD51 foci was shown (right panel). Means ± S.D. of three independent experiments are shown. *P < 0.05; **P < 0.01; ***P < 0.001 (Student's t test).

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