Table 2.
Transcriptomic and proteomic analyses of bacillithiol-related enzymes during treatment with stressors.
| Compound | Structure | Organism | Gene | Transcriptome | Proteome |
|---|---|---|---|---|---|
| Salicylic acid |  |
Bacillus subtilis 168† | yuaE (STL) | 6–8 | 5–6 |
| yojG (BshB2) | 3–4 | – | |||
| yhdN (FosB) | 3–4 | 3–4 | |||
| 2-methylhydroquinone |  |
Bacillus subtilis 168‡ | yjoA (STL) | 6 | – |
| yojG (BshB2) | 6–7 | – | |||
| yuaE (STL) | 10–14 | 4–10 | |||
| yqiW (BrxB) | 4–6 | – | |||
| 6-brom-2-vinyl-chroman-4-on |  |
Bacillus subtilis 168‡ | yjoA (STL) | 4–5 | – |
| yojG (BshB2) | 9–10 | – | |||
| yuaE (STL) | 7–10 | 3–8 | |||
| yllA (BshC) | 4 | – | |||
| yqiW (BrxB) | 4–6 | – | |||
| Hydrogen peroxide | H2O2 | Staphylococcus aureus MW2§ | MW1348 (BshA) | 0.5 | ND |
| MW2611 (BstA/STL) | 3 | ND | |||
| MW2442¶¶ (GlxA) | 2 | ND | |||
| MW1463¶¶ (GlxB) | 3 | ND | |||
| MW1467¶¶ (BrxB) | 6 | ND | |||
| Bacillus anthracis Sterne¶ | BA_1558 (BshA) | 2 | ND | ||
| BA_1557 (BshB1) | 2 | ND | |||
| BA_3524 (BshB2) | 2 | ND | |||
| BA_4058 (BshC) | 3 | ND | |||
| BA_1515¶¶ (YpdA) | 2 | ND | |||
| Diamide |  |
Staphylococcus aureus COL# | SACOL1498 (BshA) | 2 | ND |
| SACOL1190 (BshC) | 5 | ND | |||
| SACOL2717 (BstA/ STL) | 0.3 | ND | |||
| SACOL1553¶¶ (GlxB) | 2 | ND | |||
| SACOL1520¶¶ (YpdA) | 4 | ND | |||
| Nitric oxide | NO | Bacillus subtilis 168†† | yuaE (STL) | ND | 2–3 |
| Staphylococcus aureus COL†† | SACOL0614 (BshB2) | ND | 3 | ||
| SACOL1520¶¶ (YpdA) | ND | 1–3 | |||
| Nitrite | NO2− | Staphylococcus aureus N315‡‡ | SA1291 (BshA) | 3 | ND |
| SA0525 (BshB2) | 3 | ND | |||
| SA1020 (BshC) | 5 | ND | |||
| SA2310¶¶ (GlxA) | 8 | ND | |||
| SA1311¶¶ (YpdA) | 3 | ND | |||
| Azurophilic granule proteins | - | Staphylococcus aureus MW2§ | MW1348 (BshA) | 0.6 | ND |
| MW0552 (BshB2) | 0.5 | ND | |||
| MW2611 (BstA/STL) | 2 | ND | |||
| MW1463¶¶ (GlxB) | 0.7 | ND | |||
| MW1318¶¶ (BrxA) | 0.6 | ND | |||
| MW1467¶¶ (BrxB) | 2 | ND | |||
| Hypochlorous acid | HOCl | Staphylococcus aureus MW2§ | MW1348 (BshA) | 0.5 | ND |
| MW2611 (BstA/STL) | 2 | ND | |||
| MW2442¶¶ (GlxA) | 3 | ND | |||
| MW1318¶¶ (BrxA) | 0.6 | ND | |||
| MW1467¶¶ (BrxB) | 2 | ND | |||
| Mammalian peptidoglycan recognition protein (PGLYRP4) | – | Bacillus subtilis 168§§ | yuaE (STL) | 8 | ND |
Numbers < 1 show down-regulated genes, where 1 is no change.
Van Duy et al. 2007; treatment with 4 mM salicylic acid for 10 min, range shown is from duplicate experiments.
Van Duy et al. 2007; 100 µg/ml 6-brom-2-vinyl-chroman-4-on or 63 µg/ml of 2-methylhydroquinone (treatment for 10 min for both conditions), range shown is from duplicate experiments.
Palazzolo-Balance et al. 2008; treatment with 100 µg/ml azurophilic granule proteins, 5 mM H2O2, or 25 µM HOCl for 15 min for all conditions.
Pohl et al. 2011; treatment with 1.0 mM H2O2 for 10 min.
Posada et al. 2014; treatment with 1 mM diamide for 20 min.
Hochgrafe et al. 2008; treatment with 500 µM MAHMA NONOate (nitric oxide donor) 10 min, range shown is from duplicate experiments.
Schlag et al. 2007; treatment with 5 mM sodium nitrite for 6 h at 37°C.
Kashyap et al. 2014; treatment with 100 µg/ml PGLYRP4 for 30 min at 37°C.
Closest homolog to B. subtilis 168 gene.
ND: Not determined.