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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Mol Microbiol. 2016 Apr 14;100(6):1066–1079. doi: 10.1111/mmi.13368

Fig 8. Induction of FrvA increases Zn(II) resistance in a B. subtilis strain lacking Zn(II) efflux systems.

Fig 8

A. Disk diffusion assay of strains WT, Pspac-pfeT, Pspac-frvA, cadA czcD, cadA czcD Pspac-pfeT, and cadA czcD Pspac-frvA. For IPTG treated cells, 1 mM IPTG was added to both soft agar and LB agar plates. 10 μl of 100 mM ZnCl2 was added onto the filter paper disk. The data are expressed as the mean ± SD (n=3). *P<0.05 and **P<0.01 indicate a statistically significant difference between the indicated groups.

B. Levels of intracellular Zn monitored by ICP-MS for WT, cadA czcD, and cadA czcD Pspac-frvA (without and with 1 mM IPTG induction) before and at the time points indicated after addition of 250 μM ZnCl2 to LB medium. The data are expressed as the mean ± SD (n=3).