Table 1. Reverse transcriptase PCR primer sequences, annealing temperatures, and amplification product sizes for multipotent genes.

Marker	Gene	Sequence (forward/reverse)	Annealing temperature (°C)	Fragment (base pairs)
Multipotency	Sox-2	5′-TGGTTACCTCTTCCTCCCACT-3′	58.0	179
		5′-GGGCAGTGTGCCGTTAAT-3′
Housekeeping	GAPDH	5′-ACCACAGTCCATGCCATCAC-3′	60.0	450
		5′-TCCACCACCCTGTTGCTGTA-3′

Sox2, sex determining region Y-box 2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.