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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: J Urol. 2015 Dec 31;196(1):270–278. doi: 10.1016/j.juro.2015.12.081

Figure 5.

Figure 5

A. CellTracker™ fluorescent staining (green) of the mesothelial layer after 5-minute exposure of the E17 GCC followed by immediate fixation (0hr). This section shows uptake of the dye by mesothelial cells outside the peripheral muscle layer, which is delineated by myosin heavy chain (MyHC) immunostaining (red). B. Evidence for incorporation of labeled mesothelial cells (green; arrows) within deeper muscle and mesenchymal regions of the GCC after 5-minute CellTracker™ exposure followed by organ culture for 24h. Scale bar=100 μm.