Fig. 4.

Induction of jasmonates (JAs) during the early stages of Fe deficiency in roots. a Endogenous concentrations of jasmonic acid (JA) and jasmonoyl isoleucine (JA-Ile) (means ± SD, n = 3). b Transcript levels of OsDAD1;2, OsLOX2;1, OsLOX2;3, OsAOS1 and OsAOS2, genes responsible for JA biosynthesis. Non-transgenic (NT) and HRZ-knockdown (HRZi) rice were subjected to Fe sufficiency (+Fe) or deficiency (−Fe) for 6 and 24 h. Roots were harvested and used for quantification of JAs and quantitative RT-PCR. The transcript abundance was normalized against the rice α-2 tubulin transcript level and expressed as a ratio relative to the levels in the NT + Fe at 6 h (means ± SD, n = 3). Asterisks indicate significant differences compared with the +Fe NT level at each time point (*P < 0.05; **P < 0.01)