Figure 1.

EHEC infection induces hBD2 and IL-8 expression in polarized T84 cells. Polarized T84 cells were apically infected with EHEC strains EDL933, Walla-1, H07184, or left non-infected (NI) for 9 h. (A) EHEC infection did not compromise epithelial barrier function. TER after infection is expressed as percentage of TER before infection. (B) AMP and IL-8 mRNA levels were quantified by qPCR and expressed as fold changes relative to NI controls. Data are shown as means ± standard errors of the means (SEM) of four independent experiments performed in duplicate. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 vs. NI control.