Figure 3.

EHEC induction of hBD2 and IL-8 expression in polarized T84 cells is dependent on flagellin. Polarized T84 cells were apically infected with EHEC EDL933 wild-type (WT) and isogenic deletion mutants in fliC, escN, or stx (A) or EHEC 85–170 wild-type (WT) and isogenic deletion mutants in hcp or lpfA1 (B) for 9 h. IL-8 and hBD2 mRNA levels were quantified by qPCR and expressed as fold changes relative to NI controls. Data are shown as means ± SEM of three independent experiments performed in duplicate. ^***P < 0.001 vs. NI control.