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. 2016 Jun 21;6:68. doi: 10.3389/fcimb.2016.00068

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Copyright © 2016 Lewis, Prior, Ellis, Cook, Chan, Gelson and Schüller.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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EHEC stimulates hBD2 peptide expression in colonic biopsy epithelium. Colonic biopsies were incubated with EDL933, purified flagellin (1 μg/ml) or left NI for 8 h. Cryosections from snap-frozen tissue were stained with anti-hBD2 (green), and cell nuclei were counterstained with DAPI (blue). Arrowheads show epithelial cells with induced hBD2 expression. Images are displayed as separate monochrome images of each fluorescence channel (hBD2, DAPI) and merged color images from both channels (merge). Shown are representative images from two independent experiments performed in duplicate. Bars = 10 μm.