Figure 9.

EHEC-induced IL-8 release from polarized T84 cells is directional and depends on the side of exposure. Polarized T84 cells were incubated with EDL933, IL-1β (10 ng/ml), flagellin (1 μg/ml), or left NI on the apical (A,B) or basolateral side (B) for 24 h. IL-8 protein levels in cell lysates, apical and basolateral supernatants were determined by ELISA. (A) Apical exposure to EDL933 or IL-1β stimulated apical IL-8 release. Data are shown as means ± SEM of three independent experiments performed in duplicate. (B) Apical (AP) exposure to EDL933 or flagellin induced apical IL-8 release (white columns), whereas basolateral (BL) exposure to EDL933 or flagellin induced basolateral IL-8 release (black columns). Data are shown as means ± SEM of two independent experiments performed in triplicate. ^**P < 0.01, ^***P < 0.001 vs. NI control.