Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jun 21;6:64. doi: 10.3389/fcimb.2016.00064

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016 Josse, Guillaume, Bour, Lemaire, Mongaret, Draux, Velard and Gangloff.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Osteogenic differentiation (A–D). (A) Representative photographs of alkaline phosphatase staining of SM- and OM-HPBCs after 7, 14, 21, and 28 days of culture, (B) alizarin red staining of SM- and OM-HPBCs after 7, 14, 21, and 28 days of culture, and (C) expression of osteogenic genes by OM- compared to SM-HPBCs after 14 days of culture (mRNA were evaluated by RT-qPCR analysis, data were shown as specific variation in mRNA using the 2^−ΔΔCt method (HPRT-1 was used as internal control), ^* means p < 0.05 vs. SM-HPBCs). (D) Representative micrographs of 21-day immunofluorescent staining of cultured cells: type I collagen and osteocalcin were visualized in green with Alexafluor^®488. Nuclei were counterstained in blue by DAPI. Scale bar = 50 μm. Experiments were performed on four independent donors.