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. 2016 Jun 21;6:64. doi: 10.3389/fcimb.2016.00064

Figure 4.

Figure 4

Effect of S. aureus on IL-6 (A,B), IL-1β (C,D), and TNF-α (E,F) mRNA expressions by SM- (gray boxes) and OM-HPBCs (black boxes). Cells were exposed to viable S. aureus (MOI of 10:1 and 30:1) for 1, 3, 6, and 9 h in acute infection (A,C,E) and for 1, 9, 24, and 48 h in persistent infection (B,D,F). Expression of IL-6, IL-1β, and TNF-α mRNA was evaluated by RT-qPCR analysis. Data are shown as specific variation of mRNA compared to relative non-infected cells using the 2−ΔΔCt method (HPRT-1 was used as internal control). * means p < 0.05 vs. relative non-infected cells for each time period. Experiments were performed on four independent donors.