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. 2016 Jun 21;6:64. doi: 10.3389/fcimb.2016.00064

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Copyright © 2016 Josse, Guillaume, Bour, Lemaire, Mongaret, Draux, Velard and Gangloff.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of S. aureus on OPG (A,B) and RANKL (C,D) mRNAs expressions by SM- (gray boxes) and OM-HPBCs (black boxes). Cells were exposed to viable S. aureus (MOI of 10:1 and 30:1) for 1, 3, 6, and 9 h in acute infection (A,C) and for 1, 9, 24, and 48 h in persistent infection (B,D). Expressions of OPG and RANKL mRNA were evaluated by RT-qPCR analysis. Data are shown as specific variation of mRNA compared to relative non-infected cells using the 2^−ΔΔCt method (HPRT-1 was used as internal control). ^* means p < 0.05 vs. relative non-infected cells for each time period. Experiments were performed on four independent donors.