FIGURE 5.

Functional characterization of the TaTypA gene during interaction of wheat and Pst by BSMV-mediated gene silencing. (A) Mild chlorotic mosaic symptoms on the fourth leaves of seedlings inoculated with BSMV:γ, BSMV:TaPDS-as, and BSMV:TaTypA-as at 10 dpi. Mock: wheat leaves treated with 1× Fes buffer. (B) Relative transcript levels of TaTypA in TaTypA knock-down plants inoculated with CYR23. The data were normalized to the TaEF-1a gene. CK indicates BSMV:γ. Asterisks indicate significant differences from BSMV:γ using Tukey’s HSD test (P < 0.05). Photographs of fourth leaves further inoculated with urediniospores of avirulent race CYR23 (C) or virulent race CYR31 (D). Typical leaves were photographed at 15 dpi.