Fig. 3.

Optical imaging of fluorescently labelled-C1-3 detects differences in hepatic myofibroblast numbers in wild-type vs. transgenic mice after acute liver injury. (A) Whole body IVIS images and (B) graph showing average radiant efficiency in acute CCl₄ injured WT and cRel^−/− mice and oil controls. (C) Images of liver, kidney, and spleen. (D) Graph showing average radiant efficiency of ex vivo imaged CCl₄ and oil livers. (E) Photomicrographs (100× magnification, 3 × 3 fields) of αSMA staining. (F) Graph showing average αSMA+ stained area in acute CCl₄ injured and oil treated WT and cRel^−/− mice. Data are means ± s.e.m, minimum n = 8/group, scale bar, 200 μ. ANOVA, ∗p <0.05 or ∗∗∗p <0.001 compared to WT.