TABLE 1.
Strains used in the study
| Strain | MecA | Passage in: | Notes | Reference |
|---|---|---|---|---|
| E. coli | Invitrogen | |||
| RN4220 | − | Laboratory S. aureus strain | BEI Resources | |
| COLnex | − | 13 | ||
| COLnex Δpbp4 | − | pbp4 deletion mutant in COLnex | This study | |
| SF8300 | + | USA300 MRSA clinical isolate | 29 | |
| SF8300ex | − | SF8300 strain with mecA excised | 29 | |
| SF8300ex Δpbp4 | − | pbp4 deletion mutant in SF8300ex | This study | |
| SRT | − | Ceftaroline | This study | |
| SRB | − | Ceftobiprole | This study | |
| Sgap | − | SF8300ex strain in which pbp4 (E183A, F241R), gdpP (N182K), and acrB (I960V) mutations analogous to those in the CRB strain (28) were introduced | This study | |
| SgapT | − | Ceftaroline | This study | |
| Sp | − | SF8300ex strain in which pbp4 (E183A, F241R) mutations analogous to those in the CRB strain (28) were introduced | This study | |
| SpT | − | Ceftaroline | This study | |
| SRB Δpbp4 | − | pbp4 deletion mutant in SRB | This study | |
| SRT Δpbp4 | − | pbp4 deletion mutant in SRT | This study | |
| SgapT Δpbp4 | − | pbp4 deletion mutant in SgapT | This study | |
| SpT Δpbp4 | − | pbp4 deletion mutant in SpT | This study | |
| SRB Δpbp4 [empty plasmid] | − | SRB Δpbp4 with empty plasmid pTXΔ | This study | |
| SRB Δpbp4 [pbp4 (SF8300)] | − | SRB Δpbp4 complemented with pTXΔ containing pbp4 from SF8300 | This study | |
| SRB Δpbp4 [pbp4 (SRB)] | − | SRB Δpbp4 complemented with pTXΔ containing pbp4 from SRB | This study | |
| SRT Δpbp4 [empty plasmid] | − | SRT Δpbp4 with empty plasmid pTXΔ | This study | |
| SRT Δpbp4 [pbp4 (SF8300)] | − | SRT Δpbp4 complemented with pTXΔ containing pbp4 from SF8300 | This study | |
| SRT Δpbp4 [pbp4 (SRT)] | − | SRT Δpbp4 complemented with pTXΔ containing pbp4 from SRT | This study |
a +, present; −, absent.