FIG 1.

Growth kinetics of JW0452, JW0451, and JW5503 compared to those of the wild-type parent strain BW25113. The growth curves were generated by incubating 1% overnight inocula of strains BW25113, JW0452, JW0451, and JW5503 in LB medium (control) (A), LB medium supplemented with 10 mM GSH (B), LB medium with 4 ng/ml ciprofloxacin (subinhibitory ciprofloxacin concentration for BW25113) (C), LB medium with 10 mM GSH and 16 ng/ml ciprofloxacin, and LB medium with 16 ng/ml ciprofloxacin (inhibitory concentration for BW25113). The growth kinetics experiment was performed for two biological replicates. Generation times of the strains were calculated from the data of a single representative set.