TABLE 1.
Ciprofloxacin susceptibilities of wild-type E. coli BW25113 and efflux mutants in the presence and absence of GSHa
| Strain name | MIC of ciprofloxacin (ng/ml) |
GSH-mediated increase in MIC (ratio of without GSH/with GSH) (fold change) | |
|---|---|---|---|
| Without GSH | With GSH | ||
| BW25113 | 16 | 256 | 16 |
| JW5503 | 4 | 32 | 8 |
| JW0452 | 4 | 32 | 8 |
| JW0451 | 4 | 32 | 8 |
a
Only those strains where a change in MIC was observed in the presence or absence of GSH compared with that for the wild-type parent strain BW25113 are shown. The ciprofloxacin MICs for all remaining efflux pump mutants (mentioned in Table S1 in the supplemental material) were unaltered compared with that for BW25113, both in the presence and absence of 10 mM GSH.