TABLE 1.

Apparent kinetic constants for substrates of HCMV Pol^c

Substrate	Km, μM	kcat, min−1
dGTPa	0.44 ± 0.05	22 ± 0.70
GCV-TPa	5.5 ± 1.2	1.7 ± 0.17
(+)-CPV-TPb	0.51 ± 0.10	2.7 ± 0.2
(−)-CPV-TPb	15 ± 2.0	1.3 ± 0.1

^aValues for dGTP and GCV-TP are from reference 16.

^bApparent K_m values for CPV-TP enantiomers were determined by fitting data points to the Michaelis-Menten equation using GraphPad Prism (version 6). Apparent k_cat values were determined by dividing apparent V_max values by the enzyme concentrations.

^cValues are means ± standard errors on the basis of two independent replicates.