FIG 4.

Effect of different concentrations of P60.4Ac in hypromellose gel on morphology, metabolic activity, and LDH release from EMs. (A) Hematoxylin and eosin staining of EMs after 4 h exposure to 4 mg of Softisan cream, Cetomacrogol cream, or hypromellose gel containing 2% (wt/wt) P60.4Ac or no peptide. For comparison, we included P60.4Ac dissolved in PBS (100 μg/model). LDH release (B) and metabolic activity (C) of EMs after a 4-h exposure to a concentration series of P60.4Ac in hypromellose gel. The medians and ranges from experiments with cells from 3 different donors are given. *, values significantly different from those for PBS-exposed EMs.