TABLE 3.
Periplasmic domain mutants achieved from in vitro random mutagenesis and their genotypes and associated resistance phenotypesa
| AcrB region targeted by in vitro random mutagenesis | AcrB mutation(s) | Total no. of mutants with the indicated genotype | Resistance phenotype |
|---|---|---|---|
| LP1 | I38N G147D | 1 | DR |
| I38F A103T A160T | 1 | DR | |
| I38F R259S | 1 | DR | |
| I38F N144S A209E | 1 | DR | |
| I38F | 1 | DR | |
| I38S P116L A294V | 1 | DR | |
| I38F P50L T196 M | 1 | DR | |
| A22P T85A | 1 | Non-DR | |
| Y35N | 1 | Non-DR | |
| A39T | 2 | Non-DR | |
| P224L F316S | 1 | Non-DR | |
| P224T | 1 | Non-DR | |
| T85A | 1 | Non-DR | |
| LP2 | T600I I671T A752V A831T | 1 | DR |
| I671T S813P A840T | 1 | DR | |
| I671T | 3 | DR | |
| N613S I671T E693D A831V | 1 | DR | |
| I671T R699L S807P | 1 | DR | |
| V672M | 1 | Non-DR | |
| E673G | 1 | Non-DR | |
| G621S K632R Q846H | 1 | Non-DR | |
| L828F | 1 | Non-DR | |
| L828S K835N | 1 | Non-DR | |
| R586P G675A | 1 | Non-DR | |
| R717S | 1 | Non-DR | |
| K603R G621V M649V R717L F801L | 1 | Non-DR |
a
See also Tables S1 and S2 in the supplemental material. Mutations exclusively occurring within DR phenotype mutants are depicted in boldface. For definitions of the DR (boldface) and non-DR phenotypes, see Table 2.