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Overexpression of recombinant MtbTopI in M. smegmatis. (Left) Western blot analysis of mycobacterial topoisomerase I levels. The whole-cell lysates of M. smegmatis transformed with pTA-nol (lanes 1 and 2) or pTA-M+ (lanes 3 and 4) cultured with no inducer added (lanes 1 and 3) or induced with 20 ng/ml tetracycline (lanes 2 and 4) were analyzed by Western blotting using rabbit polyclonal antibodies against MtbTopI. (Right) Growth of induced cultures monitored by absorbance at 600 nm.
