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Effect of MtbTopI overexpression on rapid killing of M. smegmatis by inhibitor 2471-12. Viable colony counts from 0.1 ml of M. smegmatis transformed with either pTA-M+ (M+) overexpressing MtbTopI or pTA-nol (nol) control vector were determined prior to and following treatment with 50 μM compound 2471-12. The downward arrow indicates that no viable colonies could be detected at time points later than 8 h following addition of compound.
