TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Source (reference) |
|---|---|---|
| Strains | ||
| M. smegmatis mc2155 | ATCC | |
| Mtb:lux | M. tuberculosis CDC1551 containing the pMV306hsp+LuxG13 plasmid | This work |
| Plasmids | ||
| pKW08-Lx | Tetracycline-inducible expression plasmid | Gift from Brian Robertson (Addgene plasmid 25012) (42) |
| pTAmtop | PCR clone of topA gene coding for MtbTopI | This work |
| pTA-M+ | Luciferase gene in pKW08-Lx replaced by M. tuberculosis topA | This work |
| pTA-nol | pKW08-derived clone with no insert | This work |
| pMV306hsp+LuxG13 | Integrating E. coli-mycobacteria shuttle vector expressing an optimized luxCDABE operon | Gift from Brian Robertson and Siouxsie Wiles (Addgene plasmid 26161) (43) |