TABLE 1.

Trypanocidal activity of MPA and its derivatives

Compound	Inhibition rate (%)a
	T. congolense	T. b. brucei	T. evansi
1 (MPA)	99.60 ± 0.38	82.99 ± 2.82	90.53 ± 1.22
2	94.46 ± 3.89	5.24 ± 13.12	14.21 ± 8.64
3	2.36 ± 8.64	7.83 ± 10.35	16.66 ± 5.55
4	98.87 ± 0.78	46.13 ± 5.21	42.79 ± 4.58
5	4.65 ± 15.29	14.29 ± 34.17	32.43 ± 4.88
6	1.45 ± 10.94	22.27 ± 4.81	17.11 ± 6.14
7	4.59 ± 15.12	14.50 ± 13.76	29.44 ± 10.03
8	3.59 ± 14.06	22.99 ± 12.90	19.94 ± 8.44
9	0.06 ± 8.66	9.28 ± 5.15	11.99 ± 1.59
10	3.15 ± 8.43	9.03 ± 7.91	9.49 ± 6.13
11	6.51 ± 14.38	16.47 ± 6.97	12.79 ± 4.49
12	3.03 ± 12.91	11.56 ± 4.17	13.61 ± 8.67
Pentamidineb	99.93 ± 0.07	99.96 ± 0.06	99.94 ± 0.07
Controlc	0.00 ± 1.74	0.48 ± 1.58	−0.24 ± 2.25

^aTrypanocidal activity of MPA (compound 1) and 11 MPA derivatives (see Fig. 1) at a concentration of 1 μM was evaluated for T. congolense IL3000 strain, T. b. brucei GUTat 3.1 strain, and T. evansi Tansui strain. The inhibition rate was calculated from 3 independent experiments and expressed as the mean inhibition rate ± SD.

^bPentamidine 500 ng/ml was used as a 100% inhibition control.

^cHMI-9 medium with 0.25% dimethyl sulfoxide (DMSO) was used as a 0% inhibition control.