FIG 2.

Increase (Incr.) in the MIC by factors of 2 and frequencies of mutations observed in the replicates on which WGS was performed as a function of time (days) in the presence of the antibiotic indicated and after its removal from the growth medium. All mutations found at frequencies exceeding 0.5 were also discovered in the second replicate by PCR, except for the parC mutations in response to ciprofloxacin, where P85L in the first was replaced by S87N in the second. Piper, piperacillin; Tazo, tazobactam.