FIG 4.
μmax and maintenance energy of strains adapted to the antibiotics indicated immediately after growth in the presence of the drugs (after exposure) and after 14 days of subsequent growth in their absence (after removal). An asterisk indicates that the growth rate was significantly lower than that of the wild type, while double asterisks indicate that the growth rates before and after growth in the absence of the antibiotic differed significantly. Panels: A, μmax in duplications (Piper, piperacillin; tazo, tazobactam); B, estimation of the maintenance energy by extrapolation to D (specific growth rate in duplication per h) = 0 of the specific glucose (gluc) consumption measured at several dilution rates; C, μmax of wild-type P. aeruginosa and cells adapted to tobramycin (TBM) in the presence or absence of the drug as a function of pH.