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. 2016 Jun 7;2016:4087254. doi: 10.1155/2016/4087254

Figure 3.

Figure 3

Stalled axons upregulate PSM upon reinjury. Representative confocal images from ascending CNS axons immunolabeled for NF-m (red) in PNGs at 7 weeks after grafting ((a), (d), (g), (h), and (m), the same as Figure 2) and the reinjury group (b, e, h, k, and n). The image sets colabeled for 4EBP1 (a, b), p4EBP1 (d, e), eIF2α (g, h), peIF2α (j, k), and pS6 (m, n) are shown. Similar to Figure 2, top panel of each image set represents exposure matched orthogonal projections showing XY, XZ, and YZ planes indicating axon-specific signal (arrows). Bottom panel of each image set shows the subtracted image with axon-only signal for specific protein as indicated by an intensity spectrum. Scale bar = 5 μm. Graphs (c), (f), (i), (l), and (o) show quantification of relative immunolabeling intensity for the subtracted axon-only signals for 4EBP1, p4EBP1, eIF2α, peIF2α, and pS6, respectively. Data are expressed as fold change in signal intensity compared with 7-week grafts ± SD. ∗ p ≤ 0.05 and ∗∗ p ≤ 0.01, using Holm-Šídák post hoc test.